FIG. 1.

Microdissection of distinct anatomical regions of adult mouse lung. (A) Representative images of trachea, lung lobe, and airway tree following microdissection, original magnification 8×: (Ai) trachea and mainstem bronchi; (Aii) lung lobe, airways removed; (Aiii) airway tree immediately after isolation from the corresponding lobe; (Aiv) airway tree isolated from a different lobe; (Av) proximal airways; (Avi) distal airways dissected from the airway tree; (Avii) distal airway fragments recovered from parenchyme; (Aviii) lung parenchyme. (B) Representative western blot and (C) quantification of expression of p63, acetylated tubulin, CCSP, and pro-SPC in the isolated regions (n=3 independent experiments). Integrated optical density of protein bands was normalized to that of loading control (actin). (D) Representative images of the isolated segments (top) and corresponding regions of freshly fixed mouse lung (bottom) stained with antibodies against p63 (white), CCSP (green), and pro-SPC (red). Scale bar: D, 20 μm. Color images available online at www.liebertpub.com/scd