Amino acid sequence alignment of the SeMPNV F protein domains near the protease cleavage site with the corresponding domain of the F proteins of group I NPVs (A), dipterans (B), lepidopteran NPVs (C), group II NPVs (D), GVs (E), and errantiviruses (F). Virus and dipteran abbreviations and GenBank accession numbers of the F proteins are shown on the left and right, respectively. The conserved amino acids are boxed. The consensus sequence is shown below the alignment, in which B represents D or N, Φ indicates a hydrophobic amino acid (H.I., ≥0.12), and X represents any amino acid. A schematic presentation of the SeMNPV F protein, with the consensus sequence shown in gray boxes, is shown at the bottom. N, N terminus; C, C terminus; SP, signal peptide; TM, transmembrane domain; S-S, disulfide bridge.