FIG. 4.
PCR and restriction enzyme analyses of purified BV DNA to verify the genotype of indicated pseudotyped vAcGP64− viruses as described in the legend to Fig. 3. (A) PCR with f gene-specific primer pairs used to examine mutant viral DNAs amplifying an 834-bp fragment, when the virus contains the f gene, except for SeFΔ151-170, where a 773-bp fragment was amplified. (B) Restriction enzyme analysis of PCR-amplified DNA fragments. Mutant F genes have, in addition to the incorporated NdeI site, an additional restriction site, which is used to distinguish the viruses (Table 1). The SeFR149K mutant has neither a NdeI site nor an additional restriction site.