Figure 3. Pharmacological characterization of IBNtxA actions in vivo.

a) IBNtxA analgesia was assessed in wildtype mice and triple knockout mice at the indicated dose. The ED₅₀ values for the two groups were not significantly different. b) IBNtxA (0.5 mg/kg, s.c.) was given and analgesia assessed using the radiant heat tailflick assay in wildtype and exon 11 knockout mice. No observable analgesia could be detected in the knockout animals. c) Respiratory rate was determined in groups of mice given saline or high equianalgesic doses of morphine (20 mg/kg, s.c.)or IBNtxA (2.5 mg/kg, s.c.). d) Conditioned place preference was carried out and the activity of morphine (10 mg/kg, s.c.) and IBNtxA (1 mg/kg, s.c.) compared to saline. Results are from the literature (Majumdar et al., 2011).