TABLE 1.

Biological characteristics of Ad5 vectors carrying unliganded or liganded fibers^a

Ad5 vector	Virion titerb (PP/ml)	Infectious titerc (PFU/ml)	PP/PFU ratio
AdGFP-WTFid	1.00 × 1012	1.50 × 1011	7
AdGFP-R7-knobe	1.00 × 1012	3.75 × 109	266
AdGFP-QM10-knob	3.20 × 1011	4.00 × 109	80
AdGFP-SY12-knob	1.10 × 1012	2.88 × 1010	38
AdGFP-LAP25-knob	NAf	NA	NA

^aSamples of Ad5 vector, purified by ultracentrifugation in CsCl gradient, were assayed for infectivity and virion content.

^bThe number of virions present in each sample, comprising infectious and uninfectious virus particles, was calculated from the penton base protein content of the samples, determined by Western blot analysis (37).

^cThe titer in infectious particles was determined by plaque titration in 293 cells.

^dControl vector with WT nonliganded, long-shafted fibers.

^eControl vector with nonliganded, short-shafted fibers.

^fNA, not applicable.