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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Kidney Int. 2014 Jun 18;86(5):923–931. doi: 10.1038/ki.2014.218

Figure 2. Cyanate alters endothelial nitric oxide synthase (eNOS) protein in endothelial cells.

Figure 2

HCAECs were treated with sodium cyanate (1 or 2 mM) for 24h or 48 h. Cyanate elicits a (A) time-dependent and (B) dose-dependent decrease in eNOS protein expression. (C) Decreased phospho-eNOS in lysates from cyanate-treated cells (1 mM, 48 h) with quantification of phospho / total eNOS levels. (D) eNOS dimerization with quantification of eNOS dimer/monomer levels in lysates from cyanate-treated cells (1 mM, 48 h). Representative Western blots from HCAECs are shown. β-actin was used to normalize the data for equal protein loading and quantification of bands was done using ImageJ software. Data are expressed as mean ± SEM (n= 3-4). Statistical analysis was performed by One-Way ANOVA for more than two groups and with Student’s t-test for two groups. Significance was accepted at * p <0.05, ** p <0.01, versus control.