Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Kidney Int. 2014 Jun 18;86(5):923–931. doi: 10.1038/ki.2014.218

Figure 7. Cyanate exposure decreases eNOS in mouse aorta.

Figure 7

(A) Western blot analysis of protein from mouse aorta probed with antibodies recognizing phospho-eNOS (S1177) and total eNOS protein with (B) quantification of phospho/total eNOS levels. (C) Western blot analysis showing eNOS dimers and monomers in mouse aorta. To detect eNOS dimers and monomers, low-temperature SDS-PAGE (4°C) was performed. Shown are representative Western blots, in which bands were normalized to β-actin for equal protein loading and quantified using ImageJ software. Data expressed as mean ± SEM (n=5-7). Student’s t-test was used for comparison between groups. Significance was accepted at * p <0.05 versus control.