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. 2014 Nov;28(11):4880–4892. doi: 10.1096/fj.14-255364

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Maternal LP diet reduces the availability of ornithine in the fetal compartment and hepatic spermine concentration. A, B) Ornithine was measured in fetal plasma (A) and amniotic fluid (B) using gas chromatography-mass spectrometry (see Materials and Methods). C–E) The polyamines putrescine (C), spermidine (D), and spermine (E) were measured in lysates of fetal liver using high-performance liquid chromatography. Data are dot plots of data points from individual fetuses (A–E) with the line indicating the mean from each dietary treatment. Data were analyzed by the general linear mixed model for the fixed effects of diet, sex, and their interaction (ewe included as a random effect in the model) using Genstat 15. F) Linear discriminant plot generated after multivariate analysis of 18 amino acids in fetal plasma (circles representing 95% confidence interval around group means) conducted after normalization of data (by establishing z scores). NS, nonsignificant. Values of P < 0.05 were considered significant.