Figure 5.

Fetal kidney angiogenic potential, measured as VEGFA, is blunted by maternal LP diet. Fetal kidney angiogenic potential was indirectly determined using immunohistochemistry and quantification of cells positive for VEGFA. A) Representative micrograph of the nephrogenic zone: VEGFA staining is brown; white arrows indicate developing collecting ducts. B) Dot plot of individual data points for control and LP-exposed fetuses (control: male, n=8; females n=7; LP diet: male, n=8; female, n=8). C) Positive control, mouse kidney. D, E) Negative controls, omission of primary antibody (D) and rabbit IgG isotype control (E). All micrographs were taken at ×200. Data were analyzed by the general linear mixed model for the fixed effects of diet, sex, and their interaction term (diet×sex) using Genstat 15. NS, nonsignificant. Values of P < 0.05 were considered significant.