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. 2014 Nov;28(11):4880–4892. doi: 10.1096/fj.14-255364

Table 1.

Primer details for quantitative PCR

Primer Sequence, 5′ to 3′ TA (°C)
Angiopoietin 1 F: GGGTCACACTGGGACAGCAGG 58
R: TGGGCCACAGGCATCAAACCA
β-Actin F: TGTGCGTGACATCAAGGAGAA 55
R: CGCAGTGGCCATCTCCTG
CDH11 F: ATCTCCCCCACAGAAATCGC 54
R: CGGCCTCTTCTTCACCCATT
Cyclophilin F: CATACAGGTCCTGGCATCTTGTC 56
R: TGCCATCCAACCACTCAGTCT
GAPDH F: TCCGTTGTGGATCTGACCTG 55
R: TGCTTCACCACCTTCTTGATCTC
THOC1 F: CCATTGAACAGGCAGACCCT 54
R: GGCTTCTCCGTGCTAACAGT
Tie 2 F: CAGCAGACCTCGGAGGCAGGA 58
R: TGCCCTCTTCAGCTGCAGCATG
VEGFA F: GGATGTCTACCAGCGCAGC 56
R: TCTGGGTACTCCTGGAAGATGTC
VEGFR1 F: TGGATTTCAGGTGAGCTTGGA 52
R: TCACCGTGCAAGACAGCTTC

F, forward; R, reverse; TA, annealing temperature; CHD11, cadherin-11; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; THOC1, THO complex subunit 1; VEGFA, vascular endothelial growth factor A; VEGFR1, vascular endothelial growth factor receptor 1.