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. 2014 Nov;28(11):4880–4892. doi: 10.1096/fj.14-255364

Table 4.

Standard measureable amino acid concentrations (μm) in fetal plasma at 65 d (0.44 gestation)

Amino acid Diet
Sex
P
CP LP M F D S D × S
Alanine 285 ± 14.5 266 ± 14.5 280 ± 14.7 272 ± 14.2 NS NS NS
Glycine 397 ± 22.8 479 ± 26.2 454 ± 24.3 426 ± 24.3 0.01 NS 0.03a
Isoleucine 78.9 ± 4.44 76.1 ± 4.11 82.8 ± 4.48 72.5 ± 4.33 NS NS NS
Leucine 140 ± 6.06 127 ± 5.93 141 ± 6.06 126 ± 5.96 NS NS NS
Valine 212 ± 12.3 199 ± 11.3 216 ± 12.3 196 ± 11.7 NS NS NS
Serine 700 ± 49.1 691 ± 48.1 678 ± 47.3 717 ± 50.0 NS NS NS
Threonine 448 ± 35.3 463 ± 32.9 498 ± 34.6 417 ± 33.5 NS NS NS
Proline 119 ± 5.99 116 ± 5.57 127 ± 5.99 109 ± 5.79 NS <0.05 NS
Phenylalanine 175 ± 8.97 174 ± 8.75 187 ± 9.14 163 ± 8.99 NS NS NS
Tryptophan 87.2 ± 12.7 88.6 ± 12.7 92.0 ± 11.3 84.0 ± 11.0 NS NS NS
Tyrosine 277 ± 27.7 231 ± 22.9 269 ± 23.1 241 ± 22.4 NS NS NS
Lysine 702 ± 65.1 540 ± 61.8 662 ± 63.9 574 ± 62.8 NS NS NS
Asparagine 54.6 ± 2.87 54.1 ± 2.73 59.6 ± 2.77 49.4 ± 2.77 NS <0.05 NS
Aspartic acid 9.66 ± 0.66 9.16 ± 0.60 10.4 ± 0.66 8.41 ± 0.66 NS NS NS
Glutamic acid 218 ± 16.8 224 ± 15.9 233 ± 16.3 209 ± 16.5 NS NS NS
Total 3821 ± 179 3580 ± 170 3832 ± 174 3557 ± 174 NS NS NS

Data are mean ± sem concentrations (μM) for fetuses of ewes fed a CP diet (n=29: males, n=13; females, n=16) or an LP diet (n=31: males, n=16; females, n=15) to d 65 gestation. Data were analyzed by the general linear mixed model for the fixed effects of diet (D), sex (S), or their interaction (D×S) using Genstat 14. The ewe was included as a random term in the model. NS, nonsignificant. Values of P < 0.05 were considered significant.

a

Data illustrating the statistically significant interaction are in the Results section.