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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: J Vasc Res. 2014 Oct 15;51(4):315–324. doi: 10.1159/000367807

Figure 2. Ethanol Activates Flt-1 and Flk-1 receptors in HCAEC.

Figure 2

(a) Representative western blot data showing phospho-Flk-1 following Ethanol (25mM), VEGF (30ng) or combined Ethanol/VEGF treatment for 0 (control), 5, 10 and 30 mins. Representative western blot of (b) phospho-Flt-1 +/− EtOH, and (c) EtOH-induced p-Flk1 expression, in the absence or presence of the VEGF receptor inhibitor SU5416 (10 μM, 30 min pretreatment). (d) Phospho-AKT following Ethanol (25mM), VEGF (30ng) or combined Ethanol/VEGF treatment for 0 (control), 5, 10 and 30 mins.