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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: J Vasc Res. 2014 Oct 15;51(4):315–324. doi: 10.1159/000367807

Figure 3. Flk-1 Mediates Ethanol-stimulated Notch Signaling in HCAEC.

Figure 3

HCAEC transfected with scrambled RNA (Control), or with an siRNA targeted to Flk-1 were treated with or without EtOH (25mM, 24 h) before mRNA expression for Notch 1, 4 and Hrt-1 was determined. (a) Cumulative data for Flk-1 mRNA expression and (b) Representative western blot data in HCAEC transfected with scrambled RNA (Control), or with an siRNA targeted to Flk-1. (c) QRTPCR analysis of Notch 1, 4 and Hrt-1 mRNA expression following ethanol treatment in HCAEC transfected with scrambled RNA (Control), or with an siRNA targeted to Flk-1. Data were normalized to GAPDH and represent the mean + SEM values from four independent experiments. *P< 0.05 vs. scrambled control, #P<0.05 vs EtOH Control.