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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: J Vasc Res. 2014 Oct 15;51(4):315–324. doi: 10.1159/000367807

Figure 6. Flk-1 Mediates Ethanol–stimulated HCAEC Angiogenic Activity Via a Notch-dependent Pathway.

Figure 6

HCAEC transfected with scrambled RNA (Control), or with an siRNA targeted to Flk-1 were treated with or without EtOH (25mM, 24 h) and/or DLL4 (2.0μg, 24 h) before (A and B) network formation on Matrigel was assessed (representative images and cumulative data from 3 separate experiments conducted in triplicate was shown). (C) Notch 1 and 4, Hrt-1, Ang-1 and Tie-2 mRNA levels were analyzed by QRTPCR in HCAEC transfected with scrambled RNA (Control), or with an siRNA targeted to Flk-1 treated with or without EtOH (25mM, 24 h) and/or DLL4 (2.0μg, 24 h). Data were normalized to GAPDH and represent the mean + SEM values from three independent experiments. *P< 0.05 vs. scrambled control, #P>0.05 vs Flk-1 siRNA/EtOH.