FIG. 2.

H-111 immunoprecipitation (IP) of intracellularly expressed E1E2 heterodimers. HeLa cells infected with wild-type vaccinia virus vWA (lanes 1, 3, and 5) or vaccinia virus vWA plus vaccinia virus rHCV1-1488 (lanes 2, 4, and 6) were lysed 24 h postinfection and were immunoprecipitated with H-111, CBH-2 (positive control MAb to HCV E2, [5, 10]), or R04 (an isotype-matched negative control). The IP pellet was separated by sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis under reducing conditions, and immunoblots were analyzed with an anti-E2 MAb 3/11 (upper panel) and an anti-E1 MAb (Austral Biologicals) (lower panel). Sizes of proteins are shown in kilodaltons, and protein molecular markers are indicated on the right.