FIG. 5.

H-111 neutralization of the infectivity of HCV virions. (A) HCV virions were preincubated before the infection of Raji cells with 10 μg of H-111/ml. A negative control experiment was performed with a nonspecific HMAb (R04) and no virions. Total RNA was extracted from Raji cells 6 days after infection. HCV RNA in the cells was detected and quantitated by real-time RT-PCR. (B) Alpha interferon (IFN) inhibition of HCV virion infection of Raji cells (positive control experiment). R-sip, Raji cells expressing the sip-L gene (19). (C) E1 peptide blocks the inhibitory activity of H-111 antibody. Preincubation with H-111 and infection was performed as described for panel A except that the incubation mixture also contained an E1 (amino acids 192 to 205) peptide or a control (Ctrl) peptide.