TABLE 1.
H-111 reactivity to HCV E1 protein isolates from multiple genotypesa
| Genotype | No. of isolates | Test result for antibody
|
||
|---|---|---|---|---|
| Anti-HA (positive control) | H-111 (HMAb) | anti-CMV (negative control) | ||
| HCV 1b | 1 | +++ | +++ | − |
| HCV 1a | 3 | +++ | +++ | − |
| HCV 2b | 3 | +++ | +++ | − |
| HCV 2a | 5 | +++ | − | − |
| HCV 3a | 5 | +++ | ++ | − |
| HCV 4a | 2 | +++ | − | − |
a
HEK293 cells were transfected with HCV E1 constructs comprising E1 genes cloned from the indicated HCV genotypes and analyzed by an immunofluorescence assay (IFA). The transfected cells prepared 24 h posttransfection were fixed onto slides with acetone and stained with rat monoclonal antibody to HA at 2 μg/ml, H-111 at 5 μg/ml, or R04, a negative control antibody, at 5 μg/ml. Slides were counterstained with a 0.001% solution of Evan's blue counterstain, and bound antibody was detected with fluorescein isothiocyanate (FITC)-conjugated goat anti-human or anti-rat IgG. +++, strong; ++, moderate; −, no reactivity.