FIG. 2.

Northern analyses of the expression of the m155 transcript. At 24 h postinfection, RNA fractions were isolated from 10⁶ NIH 3T3 cells that were mock infected (lanes 1 and 5) or infected (MOI, 5) with the wild-type virus (WT) (lanes 4 and 8), Rvm155 (lanes 2 and 6), and Rqm155 (lanes 3 and 7). Equal amounts of RNA samples (25 μg) were separated on agarose gels that contained formaldehyde, transferred to a nitrocellulose membrane, and hybridized to a ³²P-radiolabeled probe that contained the sequence of M25 (M25 probe) (lanes 1 to 4) or m155 (m155 probe) (lanes 5 to 8).