FIG. 5.

Titers of MCMV mutants in the salivary glands (A), lungs (B), spleens (C), livers (D), and kidneys (E) of the CB17 SCID mice that were infected intraperitoneally with 10⁴ PFU of each virus. At 1, 3, 7, 10, 14, and 21 days postinfection, we sacrificed the animals (3 mice per group); collected the salivary glands, lungs, spleens, livers, and kidneys; and sonicated these organs. We subsequently determined the viral titers in the tissue homogenates by standard plaque assays in NIH 3T3 cells. The limit of detection was 10 PFU/ml of the tissue homogenate. The viral titers represent the average obtained from triplicate experiments. The error bars indicate the standard deviation, and those that are not evident indicate that the standard deviation was less than or equal to the height of the symbols.