FIG. 6.

The stability of the transposon insertional mutation of Rvm155 in CB17 SCID mice. We isolated viral DNAs from cells that were infected with Rvm155 (MOI < 0.01) and allowed to grow in culture for 5 days (P0) (lane 3) or from cells that were infected with the virus collected from the lungs (LU, lane 1) and salivary glands (SG, lane 2) of the infected SCID mice 21 days after intraperitoneal inoculation with 10⁴ PFU of Rvm155. Southern analyses of the viral DNA fractions digested with HindIII are shown. The ³²P-radiolabeled probe was derived from the same plasmid that was used for Southern analyses of Rvm155 shown in Fig. 1 and contained the transposon and the m155 sequence. The DNA of the wild-type virus (WT) is shown in lane 4.