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. 2014 Oct 27;15(1):132. doi: 10.1186/s12931-014-0132-z

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© Asai-Tajiri et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Th2 cytokine levels in culture supernatant were analyzed by ELISA. (A) DO11.10 spleen cells were primed with pOVA and APCs in the presence of rIL-4, anti-IL-12 antibody, CD28, and IL-2. Seven days later, CD4-positive cells were purified using MACS and used as effector T cells. CD86 siRNA-transfected BMDCs were pulsed with pOVA and stimulated with LPS and then cultured with effector T cells. (B) Coculture supernatants were collected 48 hours later, and cytokine levels were measured by ELISA (n = 4; mean ± SEM). * indicates statistically significant (P <0.05) difference between the indicated two groups.