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Construction of LANA1 point mutants. (A) Multiple sequence alignment of herpesvirus LANA1 homologues. The N-terminal amino acid sequences are aligned. The CBS and NLS of KSHV LANA1 are shown. Conserved residues are indicated by asterisks. RRV, rhesus rhadinovirus; BHV4, bovine herpesvirus 4; OHV2, ovine herpesvirus 2; HVS, herpesvirus saimiri; ORF, open reading frame. (B) Expression of FLAG-tagged LANA1 point mutants in transiently transfected 293T cells.
