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Analysis of p66 virion incorporation. M7 proviral DNA was cotransfected into 293T cells with the vpr-p51/p66 plasmids containing various mutations in the YMDD aspartates. The transfection-derived virions were concentrated by ultracentrifugation, lysed, and examined for p66 (A), p51 (B), or p24 (C) by immunoblot analysis as described in Materials and Methods.
