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. Author manuscript; available in PMC: 2014 Nov 2.
Published in final edited form as: Am J Transplant. 2013 Jul 8;13(9):2268–2279. doi: 10.1111/ajt.12342

Figure 7. Transient lymphopenia in CB conditioned allograft recipients induces the accumulation of B cells in lymph nodes and restores antibody production against allogenic antigens.

Figure 7

Figure 7

a) Flow cytometry analysis of T and B cells in the spleen and LN of naïve C57BL/6 mice, untreated C57BL/6 recipients of BALB/c cardiac allografts on day 7 post-transplant, CB-conditioned, tolerant allograft recipients on day 120 post-transplant, and CB-conditioned allograft recipients subjected to irradiation on day on day 120 post-transplant. b) Serum from naïve, untreated-rejecting allograft recipients on day 7 post-transplant, CB-conditioned, tolerant allograft recipients on day 120 post-transplant, and CB-conditioned allograft recipients subjected to irradiation on day on day 120 post-transplant was collected and tested for reactivity to BALB/c thymocytes using flow cytometry-based analyses to determine donor-reactive antibody titers. Data indicate mean titer ± SEM for n=5/group and are representative of two independent experiments. **p < 0.01