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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Cancer Res. 2014 Sep 5;74(21):6184–6193. doi: 10.1158/0008-5472.CAN-14-1357

Fig. 4.

Fig. 4

NF-κB activation is inhibited in AEG-1KO hepatocytes. A. NF-κB luciferase reporter activity was measured in WT and AEG-1KO hepatocytes. Firefly luciferase activity was normalized by Renilla luciferase activity. The activity of empty pGL3-basic vector was considered as 1. R.L.U.: Relative luciferase units. Data represent mean ± SEM of three independent experiments. *: p<0.01. B. WT and AEG-1KO hepatocytes were treated with LPS for the indicated time points and Western blotting was performed for the indicated proteins. C. Immunofluorescence followed by confocal microscopy of WT and AEG-1KO hepatocytes after LPS treatment for 30 min showing p65 nuclear translocation. D. WT and AEG-1KO hepatocytes were treated with LPS for 4 h and the mRNA level of Il1b and Il6 was measured by Taqman Q-RT-PCR. Data represent mean ± SEM of three independent experiments. *: p<0.01.