FIG. 4.

ICP0 does not modify the global acetylation activity in cells. (A) HeLa cells were infected at an MOI of 10 for 24 h by replication-defective D30EBA ICP0-overexpressing virus or treated with TSA (2 μM) for 12 h before chromatin-associated protein extraction (see Materials and Methods) and Western blotting for the detection of acetylated histone H4 (AcH4). (B) HeLa cells were transfected with indicated plasmids. At 24 h posttransfection, Western blotting was performed on cell extracts or, alternatively, acetylated proteins were immunoprecipitated with anti-acetylated lysines MAb (α-AcLys) before Western blotting. Then, 10 μg of protein was loaded for each sample on SDS-7.5% or 12.5% polyacrylamide gels for analysis. ICP0, p53, AcH4, and AcLys were detected by using MAbs 11060 and Do-7, and anti-AcH4 and anti-AcLys were detected by using rabbit polyclonal sera.