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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Cancer Res. 2014 Sep 9;74(21):6224–6235. doi: 10.1158/0008-5472.CAN-14-0819

Figure 4. [pIC]PEI induces RIG-I, MDA-5 and Noxa in PDAC cells.

Figure 4

(A) PDAC were treated with 1 µg/ml of [pIC]PEI for 48 hours. RNA was isolated, cDNA was synthesized using first strand cDNA synthesis kit and real time PCR was performed for MDA-5, RIG-I and NOXA. Results presented are normalized to GAPDH. Columns, mean of triplicate experiments; bars, S.D. * p<0.01 vs control. (B) Normal pancreatic epithelial and PDAC were treated with either [pIC]PEI or interferon-β for 48 hours. Cells were lysed and equal amounts of protein were resolved on SDS-PAGE. Blots were probed for MDA-5, RIG-I and NOXA. β-actin was used as loading control. Results are representative of three independent experiments.