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Effect of different mutations on viral infectivity. Virus stocks were obtained from the transfection of 293T cells. Stocks were normalized to the amount of p27 and used to infect LTR-SEAP-CEMx174 cells. (A) SEAP activity was measured by use of a Phosphalight kit according to the manufacturer's recommendations at 3 days postinfection. (B) SEAP activity normalized to the amount of p27.
