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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Cancer Res. 2014 Sep 12;74(21):6271–6279. doi: 10.1158/0008-5472.CAN-14-1656

Figure 1.

Figure 1

EGF-750 Probe Characterization. A, UV-Visible spectra of EGF-750 probe (red) and CF-750 dye (blue) measured to confirm that the absorbance signature of the dye is not significantly altered by conjugation. B, bioactivity of EGF-750 was verified by its ability to phosphorylate EGFR receptor tyrosine kinase in HeLa cells, measured by immunodetection of phosphorylated EGFR via Western blot with pY1068 antibody. Cells treated with either unlabeled EGF (EGF) or EGF-750 (Labeled EGF) at a concentration of 10 ng/ml showed similar levels of tyrosine kinase activation of EGFR, indicating ligand conjugation had nominal effect on probe bioactivity.