FIG. 2.

Western blot analysis of cellular chaperone molecules (Hsc70, Hsp70, and Hsp40) in HSV-1-infected cell lysates. Lane 1, molecular weight markers; lane 2, heat-shocked HeLa cell lysate; lane 3, uninfected Vero cells cultured at 37°C; lane 4, uninfected Vero cells heat shocked at 39.5°C; lane 5, uninfected Vero cells cultured at 37°C; lanes 6 to 9, HSV-1-infected cell lysates at 2, 4, 6, and 18 h postinfection, respectively. A Western blot of the viral protein ICP4 is shown as a positive control for infection, and a Western blot of α-tubulin was used as a control to ensure that similar amounts of total protein were loaded for all samples.