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. 2004 Jul;78(13):7175–7185. doi: 10.1128/JVI.78.13.7175-7185.2004

FIG. 5.

FIG. 5.

ICP0 is necessary for chaperone sequestration at early times during infection and sufficient to redistribute the cellular chaperone machinery in transfected cells. Vero cells infected with the 0β mutant (ICP0 mutant) (A to C) at 39.5°C were stained with ICP4 (green) and Hsc70 (red). This temperature was chosen because of the faster kinetics of Hsc70-enriched foci. Thus, at 6 h postexposure to HSV-1, there would have been ample time for Hsc70 focus formation. Staining for the ICP4 protein (green) (A and D) was used as a control for successful infection. As described in the text, in the absence of the ICP0 protein, no redistribution of Hsc70 was observed at this time (B), indicating that ICP0 is necessary for this action early during infection. (D to F) 0β-infected U2OS cells (a human osteosarcoma cell line that naturally complements ICP0 HSV-1 mutants) stained for ICP4 (D) and Hsc70 (E). Merged images are shown in panels C and F. Merged images of HSV-1 (strain KOS)-infected (G) and 0β-infected (H) Vero cells collected at 20 h postinfection are shown as well. (I to K) Subcellular localization of ICP0 (green) and Hsc70 (red) in cells transfected with a plasmid carrying the ICP0 gene. A merged image of the transfected cell is shown in panel K.