Fig 3.

Matrigel assay in TNBC cell lines, and T47D. Thawed 45ul Matrigel on ice were placed in each well of a pre-cooled 8-well glass chamber slide. After 30 minutes of solidification, TNBC cell lines and T47D mixed in ice-cold medium with 2% Matrigel and drugs were loaded in each well of the 8-chamber slide and incubated at 37°C. Media with 2% Matrigel and various concentrations of drugs were changed every three days. Drug concentrations were as follows; Gefitinib 1um, EMD 121463 1uM and Cetuximab 200ug/ml in MDA-MB-468, Gefitinib 1um, EMD 121463 5uM and Cetuximab 200ug/ml in HCC 1395, Gefitinib 5um, EMD 121463 2uM and Cetuximab 200ug/ml in T47D.