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Phospho-receptor tyrosine kinase array results in MDA-MB-468. After stimulation of EGF and HGF and after treatment of gefitinib and gefitinib plus EMD 121463: MDA-MB-468 in 100mm dish was harvested as described in western blotting and Phospho-Receptor Tyrosine Kinase (RTK) antibody arrays (R&D System, #ARY-001, Minneapolis, MN) were performed as recommended but with 450ug protein lysate per array.
