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. 2004 Jul;78(13):6735–6743. doi: 10.1128/JVI.78.13.6735-6743.2004

FIG. 2.

FIG. 2.

Overexpression of HDAC1 represses Tax transactivation of the integrated HTLV-1 LTR reporter in vivo. (A) β-Gal activity from a single-copy HTLV-1 LTR-lacZ reporter in pA-18G-BHK-21 cells transfected with a Tax plasmid (50 ng) (lanes 2 and 4 to 6) and an HDAC plasmid (50, 100, and 200 ng; lanes 4, 5, and 6, respectively). Lane 3 contains 200 ng of HDAC1. Cotransfection of HDAC1 represses Tax-mediated transactivation. Data presented are the mean ± standard deviation (n = 3). Lanes 4 to 6 are significantly different (P < 0.01) from results in lane 2. (B) Cotransfection of HDAC1 did not alter Tax expression. Western blot assays of Tax levels in whole-cell extracts from BHK cells transfected with Tax (50 μg) and HDAC1 (200 μg) plasmids.