Skip to main content
. 2004 Jul;78(13):6735–6743. doi: 10.1128/JVI.78.13.6735-6743.2004

FIG. 3.

FIG. 3.

HDAC1 interacts with Tax directly in vivo and in vitro. (A) Left: Western blot assay of HDAC1 and Tax levels in the immunoprecipitates following incubation of purified Flag-tagged HDAC1 and Tax proteins and immunoprecipitation with anti-Flag antibody. Right: GST-HDAC1 pull-down experiment. Tax protein was detected by anti-Tax antibody by Western blotting. Lane 2, GST control; lane 3, GST-HDAC1 pull-down; lane 1, input representing one-third of the amount added to the binding reaction mixture. (B) GST-Tax associates with HDAC1 from HeLa cell extract. Western blot analysis of HDAC1 levels from GST-Tax pull-down after incubation of HeLa cell nuclear extract with GST-Tax (lane 5) and GST control (lane 4). (C) HDAC1 interacts with Tax in vivo, as shown in Western blot assays of Tax levels in immunoprecipitates from C81 nuclear extract with an anti-HDAC1 antibody (lane 3) or a control IgG (lane 2).