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. 2014 Oct 23;5(9):774–783. doi: 10.7150/jca.9835

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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White light, NIR, and iRFP fluorescent images prior to, 8 weeks, and 11 weeks after orthotopic inoculation of SUM149-iRFP cells to the inguinal MFP. NIR fluorescent images (green) of the lymphatics were merged with iRFP fluorescent images (red) of the gene reporter in the tumor. A magnified fluorescent image of the white rectangles was also acquired. Arrow, ILN. Double arrow, ICG injection site. Double open arrow, PLN. Broken arrow, IsLN. Arrow head, SUM149-iRFP tumor. Open arrow, lymphatic capillaries due to dermal backflow. Scale, 1 mm.