FIG. 1.

Labeled particles do not exhibit cleavage and sediment similarly to procapsid NωV during velocity sedimentation. (a) Labeled and unlabeled NωV samples run on a 4-to-12% denaturing acrylamide gradient gel. Lane assignment from left to right: 1, Invitrogen SeeBlue Plus 2 molecular weight standards; 2, WT procapsid pH 7.6; 3, WT capsid pH 5.0; 4, M-NωV pH 7.6; 5, M-NωV pH 5.0. (b) Fractionation of WT procapsid, capsid, and M-NωV particles sedimented through a 10-to-40% sucrose gradient. Ten-to-40% sucrose gradients (top of gradient on the left) prepared with 250 mM NaCl, pH 7.6 buffer for WT procapsid (buffer A) and 250 mM NaCl, pH 5.0 buffer (buffer B) for M-NωV and WT capsid. The curves presented are continuous measurements of the A₂₈₀ for gradient fractionations.