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Rabbit polyclonal antibody specific for U274, the first ORF in the second largest subgenomic RNA of SARS-CoV. (A) Schematic diagram showing the predicted topology of U274; (B) Western blot analysis of various HA-tagged SARS-CoV viral proteins expressed in mammalian cells and probed with an anti-HA polyclonal antibody (upper panel) or the anti-U274 rabbit polyclonal antibody (lower panel).
