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. 2004 Jul;78(13):6818–6826. doi: 10.1128/JVI.78.13.6818-6826.2004

FIG. 4.

FIG. 4.

Oligonucleotide A or C can form a functional complex with RBP-J protein. (A) Gel shift assay of oligonucleotide A or C with purified GST-RBPJ protein. Labeled oligonucleotide A (lanes 1 to 7) or C (lanes 8 to 12) was incubated with either GST (lane 2) or GST-RBP-J (lanes 3 to 12) protein, in the absence of RBP-J oligonucleotide (lanes 3 and 8) or in the presence of unlabeled WT (10- and 40-fold) (lanes 4 and 5 and lanes 9 and 10) or mutant (10- and 40-fold) (lanes 6 and 7 and lanes 11 and 12) RBP-J oligonucleotide. G, GST protein; RP, GST-RBP-J protein. (B) Repeats of oligonucleotide A or C mediate RTA activation in a heterogeneous promoter. Oligonucleotide A or C (WT or mutant) was inserted in tandem repeats (the number of repeats is indicated) into the SV40 minimal promoter in the pGL3 promoter to drive the expression of reporter gene luciferase. The individual reporter construct was cotransfected into 293T cells with either empty vector or RTA expression vector. The fold induction by RTA was plotted, with the error bars representing standard deviations of the results from at least two independent experiments. A, WT oligonucleotide A; Am, mutant oligonucleotide A; C; WT oligonucleotide C; Cm, mutant oligonucleotide C.