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. 2014 Aug 27;307(9):C893–C899. doi: 10.1152/ajpcell.00132.2014

Fig. 2.

Fig. 2.

Serum starvation induces c-Src activity. A: c-Src activation was analyzed in A549 cells in serum-free media (0, 6 h, 24 h, 48 h) by immunoblotting for tyrosine 416 phosphorylation of c-Src (PY416-c-Src). Bottom panel: PY416-c-Src intensities were analyzed and normalized to β-actin intensities. *P < 0.05 compared with 0-h starvation data. B: A549 Phospho-c-Src Y416 analysis 24 h after exposure to a range of serum concentrations (0%, 0.5%, 1%, 5%, and 10 %) with PY416-c-Src intensities relative to β-actin intensities. *P < 0.05 compared with 0% FBS. C: immunocytochemistry of A549 cells cultured in media with or without serum for 24 h; IgG (green, top panel), PY416-c-Src (green, bottom panels), and DAPI (red) were visualized by fluorescence under equal exposure conditions. Representative immunoblots and images from 3 independent experiments are shown.