Fig. 2.
Statistical analysis of different Gα- and Gβ-subunit expression patterns in Gαi2−/− islets. Gαo1, Gαo2 (A), Gαs(long), Gαs(short) (B), and Gαq (C) expression levels are not affected by Gαi2 deletion in Gαi2−/− islets. D: Gαi3 protein upregulation is detectable in Gαi2−/− islets. To verify Gαi3 antibody specificity, Gαi3−/− islets were loaded. E: Gβ1 and Gβ2 protein levels are downregulated in Gαi2−/− islets. Gβ2 downregulation gains statistical significance. Insets: representative immunoblots of islet homogenates from wt and Gαi2−/−. Equal loading was confirmed by β-actin or α-tubulin detection.