Fig. 6.

Morphometric analyses of Gα_i2^βcko islets. A: representative H&E-stained ctrl and Gα_i2^βcko islets. B: representative sections of pancreata from ctrl and Gα_i2^βcko mice visualized by immunofluorescence with anti-insulin (red) and anti-glucagon (green) antibodies. Scale bar, 40 μm. Islet size (C), number of nuclei per islet (D), and mean cell size (E) of ctrl and Gα_i2^βcko islets were equal. Morphometric analyses were performed in 6-wk-old male mice. In total, 381 control and 455 Gα_i2^βcko islets derived from 3 mice per genotype were quantified for this evaluation. Whole pancreas insulin content (F), islet insulin (G), and islet DNA content (H) of ctrl and Gα_i2^βcko mice did not differ between genotypes. Islet insulin or islet DNA content was determined following static incubation experiments (n, number of triplicates).