Fig. 1.

Steroid receptor coactivator 1 (SRC1) antisense oligonucleotide (ASO) specifically decreases SRC1 expression in the liver and white adipose tissue (WAT). Sprague-Dawley rats were treated with control or SRC1 ASO for 4 wk, and mRNA expression was analyzed by real-time PCR in euglycemic hyperinsulinemic-clamped rats. A–C: SRC1 expression in the liver, WAT, brown adipose tissue (BAT), and muscle (A), SRC2 (B), and SRC3 expression (C) in the liver and WAT. D: real-time PCR analysis of hepatic gluconeogenic gene expression in overnight-fasted rats; n = 6–8/group. P value calculated by Student's t-test. *P < 0.05. GP6C, glucose 6-phosphatase catalytic; G6PT, glucose 6 phosphatase translocase; FBPase, fructose-1,6-bisphosphatase; PC, pyruvate carboxylase; PEPCK1, phosphoenolpyruvate carboxykinase 1.