Fig. 2.

Protein synthesis in gastroc complex (gastrocnemius, soleus, and plantaris, i.e., mixed skeletal muscle), heart, and liver over 2 wk in 4-mo and 7-mo CL and Con. There were no differences in protein synthesis between groups in any tissue between 4-mo CL and 4-mo Con (A, C, and E). Protein synthesis was significantly higher in all tissues and fractions in 7-mo CL vs. 7-mo Con (B, D, and F); n = 4 per group and fraction except Con liver Mix n = 3 because of an unquantifiable peak on the GC. Two values in liver 7-mo CL Cyto and Mito were fully turned over (i.e., 100% new) after 2 wk, and so calculations were performed with a synthesis value of 1.00 for those animals. *P < 0.05 for CL vs. Con; no. P < 0.05 difference between pooled (Con and CL) fractions in all tissues at both 4 and 7 mo.