Fig. 6.

A–F: Western blotting of mTORC1 substrates rpS6 and 4E-BP1 in 7-mo CL mice. rpS6 phosphorylation was significantly greater in heart and liver in 7-mo CL than in Con (B and C). 4E-BP1 phosphorylation was significantly greater in gastroc complex and tended to be greater in heart (P = 0.055) in 7-mo CL vs. 7-mo Con (D and E). Data are expressed as ratio of phosphorylated to total protein. β-Tubulin is shown to verify equal loading of protein. Liver rpS6 was log transformed to normalize variance; n = 4 per group. *P < 0.05 for CL vs. Con.