Fig. 2.

Renal I/R causes PAD4-mediated citrullination of nuclear histone H3. Images are representative of four immunohistochemistry experiments performed to stain for citrullinated histone H3. Kidneys subjected to renal I/R showed increased nuclear citrullinated histone H3 staining (dark brown, ×1,000) compared with those from sham mice. Mice treated with the selective PAD inhibitor 2-chloroamidine (2-Cl-amidine) showed markedly attenuated nuclear citrullinated histone H3 staining after renal I/R. Kidneys incubated with negative isotype control antibody showed no staining in the nucleus (n = 4 experiments).