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Evaluation of fibrosis in liver sections. There was enhanced fibrosis (evaluated by Sirius red staining) and increased expression of α-smooth muscle actin (α-SMA) in BDL compared with normal rats. When BDL rats were exposed to complete dark for 1 wk, there was reduced fibrosis and expression of α-SMA compared with BDL rats exposed to light-dark cycles. Original magnification, ×40.
