Table 1.
Measurement of liver, body weight and liver-to-body weight ratio; serum chemistry; % PCNA-positive cholangiocytes and IBDM; and melatonin serum levels
| Parameters | Normal + Light-Dark Cycles 1 wk | Normal + Complete Dark 1 wk | BDL + Light-Dark Cycles 1 wk | BDL + Complete Dark 1 wk |
|---|---|---|---|---|
| Liver weight, g | 8.7 ± 0.2 | 9.0 ± 0.6 | 9.8 ± 0.1* | 8.7 ± 0.5† |
| (n = 9) | (n = 7) | (n = 8) | (n = 12) | |
| Body weight, g | 217.7 ± 5.8 | 226.0 ± 5.1 | 170.2 ± 4.3* | 175.0 ± 2.7 |
| (n = 12) | (n = 7) | (n = 12) | (n = 12) | |
| Liver-to-body weight ratio, % | 4.30 ± 0.2 | 3.9 ± 0.2 | 5.6 ± 0.1* | 4.9 ± 0.3† |
| (n = 12) | (n = 7) | (n = 12) | (n = 12) | |
| Serum melatonin levels, pg/ml | 61.52 ± 8.71 | 207.0 ± 84.1 | 110.1 ± 18.4* | 145.3 ± 47.5 |
| (n = 15) | (n = 17) | (n = 15) | (n = 12) | |
| SGPT, U/l | 72.7 ± 12.1 | 156.1 ± 32.5* | 396.3 ± 95.6* | 115.7 ± 13.9† |
| (n = 9) | (n = 8) | (n = 10) | (n = 4) | |
| SGOT, U/l | 185.9 ± 24.5 | 270.0 ± 58.4‡ | 1,105.8 ± 245.4* | 249.2 ± 25.1† |
| (n = 12) | (n = 8) | (n = 11) | (n = 4) | |
| Total bilirubin, mg/dl | <0.1 | <0.1 | 11.5 ± 1.2* | 4.7 ± 1.3† |
| (n = 7) | (n = 6) | (n = 12) | (n = 4) | |
| ALP, U/l | 276.4 ± 13.7 | 244.2 ± 9.1‡ | 400.63 ± 33.0* | 357.5 ± 23n.s. |
| (n = 8) | (n = 5) | (n = 10) | (n = 4) | |
| Volume fraction occupied by collagen (Sirius red-stained) fibers | 1.8 ± 0.2 | 1.9 ± 0.2 | 6.3 ± 1.5* | 3.5 ± 0.6† |
| α-SMA+ portal MF/HSC (cells/HPF) | 1.5 ± 0.3 | 1.0 ± 0.26 | 9.7 ± 1.4 | 6.6 ± 0.7 |
*
P < 0.05 BDL vs. normal rats.
†
P < 0.05 BDL + dark vs. BDL;
‡
not significant vs. the corresponding rats exposed to dark-light cycles.
BDL, bile duct ligation; IBDM, intrahepatic bile duct mass; SGOT, serum glutamic oxaloacetic transaminases; SGPT, serum glutamate pyruvate transaminases; ALP, alkaline phosphatase; α-SMA, α-smooth muscle actin, MF, myofibroblasts; HSC, hepatic stellate cells; HPF, high-powered field.