Fig. 4.

Calcitriol induces ileal FGF15 and hepatic SHP pathway to suppress lipogenic machinery and module enterohepatic circulation in the physiological condition. Balb/C mice (n = 8) were given a single dose of calcitriol or control. A: ileal expression of FGF15, FXR, and ASBT was determined by qRT-PCR analysis. B: machinery for hepatic lipogenesis, including FXR, SHP, SREBP-1c, and FAS expressed as the mRNA in the liver, was measured by qRT-PCR analysis. C: Cyp7A1 and Cyp3A11, for bile acid synthesis and catabolism, respectively, in the form of mRNA levels expressed in the liver, were determined by qRT-PCR analysis. D: total bile acids in the liver and gallbladder were determined. The data are presented as means ± SE. *P < 0.05, **P < 0.01.