Fig. 5.

Manipulating enterohepatic circulation by bile acid sequestration (BAS) attenuates steatosis. Balb/C mice (n = 8) were fed with HFD or not for 2 wk, followed by addition of cholestyramine to the diet or not for a consecutive 6 wk. A: representative slides of H&E staining and Oil-Red-O staining. B: TG levels in the liver tissues, in milligrams per gram of liver weight. C: body weight of the subjects. D: total bile acid levels in the liver and gallbladder, in micromoles per gram of liver weight and micromoles per gram of body weight, respectively. E: SHP, SREBP-1c, FAS, Cyp7A1, TNF-α, and IL-6 expressed in the liver as mRNA were determined by qRT-PCR analysis. F: FGF15 and ASBT expressed in the ileum as the mRNA levels were determined by qRT-PCR analysis. *P < 0.05, **P < 0.01.